Journal:

Article Title: The ADP Ribosyltransferase Domain of Pseudomonas aeruginosa ExoT Contributes to Its Biological Activities

doi: 10.1128/IAI.72.1.546-558.2004

Figure Lengend Snippet: Yeast strains and plasmids used in this study

Article Snippet: Yeast strains and expression plasmids used in this study are summarized in Table . table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Strain or plasmid Relevant characteristics Source or reference AH109 MAT a trp1-901 leu2-3 , 112 ursa3-52 his3-200 gal4 Δ gal80 Δ LYS2 :: GAL1 UAS -GAL1 TATA -HIS3 GAL2 UAS -GAL2 TATA -ADE2 URA3 :: MEL1UAS-MEL1TATA-lacZ 27 L4852 MAT a can1-100 GAL + ura3-1 leu2-3 , 112 his3-11 , 15 ade2-1 trp1-1 pGBKT7 Vector for constitutive expression in yeast Clontech pGBKT7-GAP GAP domain of ExoT cloned into the Sal I site of pGBKT7 This study pGBKT7-GAP(R149K) GAP domain of ExoT with a R149K mutation subcloned into the Sal I site of pGBKT7 This study pGBKT7-ADPRT ADPRT domain of ExoT cloned into the Eco RI site of pGBKT7 This study pGBKT7-ADPRT(AAA) ADPRT domain of ExoT with an EQE383-385AAA mutation subcloned into the Eco RI site of pGBKT7 This study pRS424-GAL1 Vector for galactose-inducible expression 36 pRS424-GAL1/GAP GAP domain of ExoT subcloned into the Eco RI site of pRS424-GAL1 This study pRS424-GAL1/GAP(R149K) GAP domain of ExoT with a R149K mutation subcloned into the Eco RI site of pRS424-GAL1 This study pRS424-GAL1/ADPRT ADPRT domain of ExoT subcloned into the Eco RI site of pRS424-GAL1 This study pRS424-GAL1/ADPRT(AAA) ADPRT domain of ExoT with an EQE383-385AAA mutation subcloned into the Eco RI site of pRS424-GAL1 This study Open in a separate window Yeast strains and plasmids used in this study The wild-type and mutant versions of the GAP and the ADPRT domains of ExoT were also subcloned as Eco RI fragments into the inducible yeast vector pRS424-GAL1 ( 36 ).

Techniques: Plasmid Preparation, Expressing, Clone Assay, Mutagenesis